A8967

Immunoblotting
Rat brain cytosolic S1 fraction was separated on SDS-PAGE and probed with IgG Fraction AB Amyloid Precursor Protein ) (Cat. No. A8967). The antibody was developed with Alk’ Phos’ APA Rabbit IgG (Cat. No. A9919) and NBT/BCIP substrate.
Antibody dilution 1/2,000.

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A8967

Sigma

 

Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym:Anti-APP
MDL number:MFCD02095830

Description

Immunogensynthetic peptide corresponding to the N-terminal of human APP695 (amino acids 46-60) conjugated to KLH.
SpecificityThe immunogen sequence is identical to the APP isoforms APP751 and APP770 and is highly conserved (single amino acid substitution) in rat and mouse APP695. The antibody recognizes APP695, APP751 and APP770.
Physical formSolution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody formIgG fraction of antiserum
clone(polyclonal)
formbuffered aqueous solution
mol wtantigen mol wt 95-100 kDa
species reactivityrat, mouse, human
application(s)immunoblotting: 1:1,000 using rat brain extract
 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formic acid-treated sections of human Alzheimer′s disease (AD) brain
 microarray: suitable
Gene Informationhuman ... APP(351)
 rat ... App(54226)
 mouse ... App(11820)
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3

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References

referenceAndreasson, K., et al., Deletion of the prostaglandin E2 EP2 receptor reduces oxidative damage and amyloid burden in a model of Alzheimer’s disease. J. Neurosci. 25, 10180-10187, (2005) Abstract
 Annex, B.H., et al., Oxidative phenotype protects myofibers from pathological insults induced by chronic heart failure in mice. Am. J. Pathol. 170, 599-608, (2007) Abstract